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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
23/07/1996 |
Data da última atualização: |
23/07/1996 |
Autoria: |
BAU, S. A.; LIMA, M. R. de. |
Título: |
Variabilidade de algumas caracteristicas de um latossolo vermelho amarelo pase cerrado cultivado e nao cultivado. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
IN: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 21. Petrolina, PE, 1994. Anais. Petrolina: EMBRAPA-CPATSA / SBCS, 1994. p. 282-283 |
Idioma: |
Português |
Palavras-Chave: |
Latossolos; Solos. |
Thesagro: |
Cerrado; Manejo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00595naa a2200169 a 4500 001 1365453 005 1996-07-23 008 1994 bl uuuu u00u1 u #d 100 1 $aBAU, S. A. 245 $aVariabilidade de algumas caracteristicas de um latossolo vermelho amarelo pase cerrado cultivado e nao cultivado. 260 $c1994 650 $aCerrado 650 $aManejo 653 $aLatossolos 653 $aSolos 700 1 $aLIMA, M. R. de 773 $tIN: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 21. Petrolina, PE, 1994. Anais. Petrolina: EMBRAPA-CPATSA / SBCS, 1994. p. 282-283
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Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
08/03/2010 |
Data da última atualização: |
03/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
FUGANTI, R.; MACHADO, M. de F. P. da S.; LOPES, V. S.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; ABDELNOOR, R. V.; MARCELINO, F. C.; NEPOMUCENO, A. L. |
Afiliação: |
RENATA FUGANTI, UEM; MARIA DE FÁTIMA PIRES DA SILVA MACHADO, UEM; VALÉRIA STEFANIA LOPES, UNIFIL; JOAO FLAVIO VELOSO SILVA, CPAMT; CARLOS ALBERTO ARRABAL ARIAS, CNPSo; SILVANA REGINA ROCKENBACH MARIN, CNPSo; ELISEU BINNECK, CNPSo; RICARDO VILELA ABDELNOOR, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo. |
Título: |
Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Biomedicine and Biotechnology, 2010. |
Páginas: |
9 p. |
ISSN: |
1110-7251 |
DOI: |
10.1155/2010/847673 |
Idioma: |
Inglês |
Conteúdo: |
During earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. |
Palavras-Chave: |
Marcadores moleculares; Nematóide de galha; Soybean. |
Thesagro: |
Meloidogyne Javanica; Nematóide; Soja. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162362/1/847673-1.pdf
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Marc: |
LEADER 02071naa a2200337 a 4500 001 1660121 005 2017-08-03 008 2010 bl uuuu u00u1 u #d 022 $a1110-7251 024 7 $a10.1155/2010/847673$2DOI 100 1 $aFUGANTI, R. 245 $aSize of AT(n) insertions in promoter region modulates Gmhsp17.6-L gene expression levels. 260 $c2010 300 $a9 p. 520 $aDuring earlier experiments, an SSR molecularmarker (176 SoyHSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number ofAT(n) insertions inside this promoter region couldmodulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. 650 $aMeloidogyne Javanica 650 $aNematóide 650 $aSoja 653 $aMarcadores moleculares 653 $aNematóide de galha 653 $aSoybean 700 1 $aMACHADO, M. de F. P. da S. 700 1 $aLOPES, V. S. 700 1 $aSILVA, J. F. V. 700 1 $aARIAS, C. A. A. 700 1 $aMARIN, S. R. R. 700 1 $aBINNECK, E. 700 1 $aABDELNOOR, R. V. 700 1 $aMARCELINO, F. C. 700 1 $aNEPOMUCENO, A. L. 773 $tJournal of Biomedicine and Biotechnology, 2010.
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Embrapa Soja (CNPSO) |
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